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Edvotek 225 Usage of Restriction Enzymes in DNA Fingerprinting Analysis, For 6 Gels

Quick recovery of ultra-pure DNA from agarose gels.
Column design permits DNA elution at high concentrations into minimal volumes.
Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.

Clean and concentrate (e.g., PCR purification) up to 25 µg DNA in as little as 25µl elution in 2 minutes with 0µl wash residue carryover.
Column design allows DNA to be eluted at high concentrations into minimal volumes as little as 25ul.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
DNA Recovery: As much as 25ug. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%..

dATP, dCTP and dTTP also available with us..
Applications PCR amplification of DNA fragments DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.

dATP, dGTP and dTTP also available with us..
Applications PCR amplification of DNA fragments DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.

Box contains contains 1 ml of 2xMasterMix, 25 mM MgCl2, nuclease free water and DMSO. 2XMasterMix is premixed with sample loading dye for immediate loading after the PCR reaction..
Stable at room temperature. Long term storage at -20^oC required..
Applications PCR amplification of DNA fragments up to 10 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR..

Contains 1 ml of 2xMasterMix, 25 mM MgCl2, nuclease free water and DMSO. 2XMasterMix is premixed with sample loading dye for immediate loading after the PCR reaction..
HotStart PCR for specific amplification. Comparable with the Platinum Taq polymerase. Good for TA cloning..
Dye Minus HotSatrt 2X Master Mix is also available upon request..
Applications PCR amplification of DNA fragments up to 10 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.

Isolate genomic DNA from both Gram-positive & Gram-negative bacteria.
High yield, high quality DNA for sensitive downstream applications including sequencing, PCR & qPCR.
Spin columns-collection tubes- 2 mL (50/bag).

Storage Buffer 20mM Tris-HCl, 1mM DTT, 0.1mM EDTA,100mM KCl 50% glycerol, 0.5%NP40, 0.5%TW20.
10×PCR Buffer 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM KCl, 50 mM (NH4)2SO4, 15mM MgCl2, 0.5% NP-40.
Unit definition One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74°C under assay conditions..
Applications PCR amplification of DNA fragments up to 5 kb DNA labeling and DNA sequencing PCR for cloning. Quality Control No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.

Kit contains (4) DF columns, (4) 2mL collection tubes, 3mL DF buffer, 1mL wash buffer (4mL add ethanol) and 1mL elution buffer.
Sample: up to 300mg agarose gel slice and up to 100 microliter PCR product or other enzymatic reaction.
expectant yield is 80-90 percent for gel extraction and 90-95 percent for PCR clean-up.
Applications: PCR fluorescent or radioactive sequencing, restriction digestion, DNA labeling, ligation.

Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.

Designed for use on the majority of PCR, qPCR and sequencing instruments with 384 well block.
Compatible with robotic systems and standard multichannel pipettes.
fit for use with ABI/LifeTechnologies qPCR instruments.
Frame: PC, rigid for added mechanical stability & improved seal integrity.
Wells: PP, thin-walled for optimum PCR/qPCR results.
003c30 µl working volume, 55 µl total well capacity.
Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen.

PROBIOTIC FOR DIGESTIVE HEALTH: Take daily to help maintain good colon health, relieve occasional digestive discomfort and promote healthy immune....
TRUSTED PROBIOTIC SUPPLEMENT: No Refrigeration Required. Non-GMO, Gluten Free, Dairy Free. DNA Sequencing Verified for each strain. Stomach....
BALANCED DIGESTIVE SUPPORT: This probiotic is specifically formulated to maintain good colon health, relieve occasional digestive discomfort, promote....
THE FRIENDLY TRIO BACTERIA: Our proprietary blend of three clinically studied strains, L. gaseri, B. bifidum and B. longum, that have been shown to be....
EXPLORE WAKUNAGA: With a mission to promote good health throughout the world, Wakunaga offers nutritional supplements including Kyolic Aged Garlic....

PROBIOTIC WITH ENZYMES: Contains four key digestive enzymes that may assist the body’s natural ability to break down proteins, fats, carbohydrates....
TRUSTED PROBIOTIC SUPPLEMENT: No Refrigeration Required. Non-GMO, Gluten Free, Dairy Free. DNA Sequencing Verified for each strain. Stomach....
BALANCED DIGESTIVE SUPPORT: This probiotic is specifically formulated to maintain good colon health, relieve occasional digestive discomfort, promote....
THE FRIENDLY TRIO BACTERIA: Our proprietary blend of three clinically studied strains, L. gaseri, B. bifidum and B. longum, that have been shown to be....
EXPLORE WAKUNAGA: With a mission to promote good health throughout the world, Wakunaga offers nutritional supplements including Kyolic Aged Garlic....